| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Thermosipho melanesiensis | AGACACACGTGGAGGAACAAT | AGTTTGTTGTATGGTGCGGAT | 59.58 | 58.21 | 240 | 128 |
100.00%
|
100.00%
|
| Primer 52 | Thermosipho melanesiensis | GACACACGTGGAGGAACAAT | AGTTTGTTGTATGGTGCGGAT | 58.12 | 58.21 | 239 | 128 |
100.00%
|
100.00%
|
| Primer 53 | Thermosipho melanesiensis | TGGCTGGAAGAAGGGCAT | GCGTTAACTCCCAAAGGATCC | 58.49 | 58.99 | 176 | 127 |
100.00%
|
100.00%
|
| Primer 54 | Thermosipho melanesiensis | CACGGTGTAGGAGCTTTAGCA | ACCCACACTACCGCAAACA | 60.07 | 59.47 | 290 | 126 |
100.00%
|
100.00%
|
| Primer 55 | Thermosipho melanesiensis | GCCCTGGGACCATATGTAGG | CGCACTTGGTGGCATTACC | 59.31 | 59.49 | 249 | 126 |
100.00%
|
100.00%
|
| Primer 56 | Thermosipho melanesiensis | CCCAGAACTGTTGCACAAGC | TCCCCACCACATACATCACAC | 59.97 | 59.72 | 209 | 125 |
100.00%
|
100.00%
|
| Primer 57 | Thermosipho melanesiensis | CCCAGAACTGTTGCACAAGC | ACTCCCCACCACATACATCAC | 59.97 | 59.44 | 211 | 125 |
100.00%
|
100.00%
|
| Primer 58 | Thermosipho melanesiensis | CCCAGAACTGTTGCACAAGC | CCCCACCACATACATCACAC | 59.97 | 58.25 | 208 | 125 |
100.00%
|
100.00%
|
| Primer 59 | Thermosipho melanesiensis | GCAACGGGAATGTCAGCAC | TCTGCATCGGGATGTGTAGG | 59.79 | 59.25 | 236 | 125 |
100.00%
|
100.00%
|
| Primer 60 | Thermosipho melanesiensis | ACAGCTGGTGATGGGGAGA | CTTCTCCTTCTTCGCCACCA | 60.23 | 59.68 | 238 | 125 |
100.00%
|
100.00%
|